Introduction: The Primo initiative aims to monitor and prevent antibiotic resistance and healthcare-associated infections in community healthcare settings and the medical-social sector. The 2020 national data on antibiotic resistance in community settings and nursing homes focus on three bacterial species of interest (Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus). Method: From January 1 to December 31, 2020, susceptibility testing results for E. coli, K. pneumoniae, and S. aureus isolated from diagnostic specimens were collected from a network of 1,311 clinical laboratories (LBM) spread across the 13 regions of mainland France and Martinique. Antibiograms of strains isolated from patients living at home or residing in nursing homes were included in the analysis. Screening samples and samples from healthcare facilities were excluded. Departmental incidence rates of urinary E. coli strains producing ESBL were estimated. Trends in the percentage of these strains were compared within a cohort of MBFs before and after the first lockdown implemented in March 2020 to curb the COVID-19 pandemic. Statistical analyses were performed using Student’s t-test or analysis of variance. Results: In 2020, 584,183 antibiotic susceptibility tests were performed on Enterobacteriaceae strains isolated from urinary specimens collected by the Primo and Spares initiatives (of which 86.1% were E. coli and 9.1% were K. pneumoniae). Among patients living at home, 3.3% of the isolated E. coli strains were resistant to third-generation cephalosporins (C3G) and 3.0% produced extended-spectrum beta-lactamases (ESBLs). Among nursing home residents, resistance to 3G was observed in 10.2% of E. coli strains, and 9.2% were ESBL-producing. In outpatient care, the percentage of ESBL-producing E. coli decreased significantly after the first lockdown (3.1% before vs. 2.9% after, p